Sunday, January 19, 2014

Tools for molecular neuroanatomy

This is just a quick listing of tools I often recommend to students at the Course in Molecular Neuroanatomy. They are mainly for dealing with many genes.

From slides:


Finding out when and where genes are expressed:

  • Excross
    • mouse genes as input
    • returns when and where a list of genes is expressed
    • find out which genes are in other Allen datasets
    • wait for images to load
    • reload page for new input
  • HBAset
    • specific expression for many genes in the 6 Allen HBA donors
    • returns where a list of genes are specifically expressed
    • under construction
    • treeview button doesn't work right in chrome, try firefox
I made the above two so if you find them helpful or are using them for analysis then contact me for extensions and help.

Lapis
  • extracting gene symbols from text
  • visual xml parsing, method:
    • click on the edit simultaneously button
    • select gene symbol or text of interest
    • copy and paste into a new file inside lapis
    • copy again and paste in excel or somewhere else

VennMaster
  • comparing gene lists
  • making venn/Euler diagrams
  • input is a text file with tab separated gene then group entries
    • use a spreadsheet tool to make list, then paste into text file
  • use control+c to copy genes in mac osx
  • used to test if the overlap between two gene sets is significant

Gene ontology analysis
Gene network



  • easiest to just set input+output to gene symbol

Extracting gene lists from PDF files - pdftohtml maybe useful. 

Thanks to Christos Gkogkas for suggesting I keep track of these.